pBbA5c-MevT(CO)-MBIS(CO, ispA)
Ancestors
Main features
p15A ori, Ptrc, atoB, hmgs, hmgr, erg12, erg8, mvd1, idi, and ispASelection marker:
Kanamycin
Modifications
| Gene | Organism | Modification |
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Strains harboring plasmid pBbA5c-MevT(CO)-MBIS(CO, ispA)
- Escherichia coli DH5αZ1 (pZE-wvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
- Escherichia coli DH5αZ1 (pZE-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
- Escherichia coli DH5αZ1 (pTrcHis-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
- Escherichia coli BL21(DE3)/(pET-wvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
- Escherichia coli BL21(DE3)/(pET-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
References
- Nybo, S. Eric; Saunders, Jacqueline & McCormick, Sean P. Metabolic engineering of Escherichia coli for production of valerenadiene. Journal of Biotechnology. 2017, 262, 60-66.