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pY1

Ancestors
  • All plasmids
    • pRBS01
      • pY1



Main features
p15A ori, Plac-UV5-tyrB-tyrA*-aroC-aroA-aroL

Selection marker:

Ampicillin



Modifications
Gene Organism Modification
tyrB
Escherichia coli
Overexpression
tyrA
Escherichia coli
Feedback insensitive
aroC
Escherichia coli
Overexpression
aroA
Escherichia coli
Overexpression
aroL
Escherichia coli
Overexpression



Strains harboring plasmid pY1
  • Escherichia coli C (pS4, pY1)
  • Escherichia coli D (pS5, pY1)

References
  • Darmawi Juminaga, Edward E. K. Baidoo, Alyssa M. Redding-Johanson, Tanveer S. Batth, Helcio Burd, Aindrila Mukhopadhyay, Christopher J. Petzold & Jay D. Keasling (2011). Modular Engineering of l-Tyrosine Production in Escherichia coli. Applied and Environmental Microbiology.

Reference

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