pETM6R1-EcCA_RBS03-KpcPA*_RBS07-KpcPD_RBS22-PfeGT_RBS22-PfeGD_RBS22

Ancestors

All plasmids

pETM61

pETM6R1-EcCA_RBS03-KpcPA*_RBS07-KpcPD_RBS22-PfeGT_RBS22-PfeGD_RBS22

Main features

pBR322 ori, P_Trc-EcCA_RBS03-KpcPA*_RBS07-KpcPD_RBS22-PfeGT_RBS22-PfeGD_RBS22-T_T7

Selection marker:

Ampicillin

Modifications

Gene	Organism	Modification
*cadA*	Escherichia coli	Overexpression
*patA*	Klebsiella pneumoniae	Overexpression
*KpcPD*	Klebsiella pneumoniae	Overexpression
*gabT*	Pseudomonas fluorescens	Overexpression
*gabD*	Pseudomonas fluorescens	Overexpression

Strains harboring plasmid pETM6R1-EcCA_RBS03-KpcPA*_RBS07-KpcPD_RBS22-PfeGT_RBS22-PfeGD_RBS22

References

Jiaping Wang, Cong Gao, Xiulai Chen & Liming Liu (2021). Engineering the Cad pathway in Escherichia coli to produce glutarate from l-lysine. Applied Microbiology and Biotechnology.