pETM6R1-EcCA_RBS22-KpcPA_RBS22-KpcPD_RBS22

Ancestors

All plasmids

pETM61

pETM6R1-EcCA_RBS22-KpcPA_RBS22-KpcPD_RBS22

Main features

pBR322 ori, P_Trc-EcCA_RBS22-KpcPA_RBS22-KpcPD_RBS22-T_T7

Selection marker:

Ampicillin

Modifications

Gene	Organism	Modification
*cadA*	Escherichia coli	Overexpression
*patA*	Klebsiella pneumoniae	Overexpression
*KpcPD*	Klebsiella pneumoniae	Overexpression

Strains harboring plasmid pETM6R1-EcCA_RBS22-KpcPA_RBS22-KpcPD_RBS22

Escherichia coli AVA-01

References

Li, Xiaoxi; Yu, Wenwen; Guo, Baoyuan; Lang, Xutao; Xu, Xianhao; Liu, Yanfeng; Li, Jianghua; Du, Guocheng; Lv, Xueqin & Liu, Long. Metabolic engineering of Escherichia coli for biosynthesis of inosinic acid. Systems Microbiology and Biomanufacturing. 2025, 5(4), 1609-1621.