Escherichia coli
aroG
Sequence
aroG encodes 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase (also known as 2-dehydro-3-deoxyphosphoheptonate aldolase), the first committed and rate-limiting enzyme of the shikimate pathway, catalyzing the condensation of phosphoenolpyruvate and erythrose 4-phosphate to form DAHP. Native AroG activity is subject to feedback inhibition by aromatic amino acids; therefore, expression of feedback-resistant variants has been widely employed to increase intracellular aromatic precursor availability and enhance tyrosine and downstream flavonoid production in Escherichia coli [351,360].
In particular, the feedback-resistant mutant aroGfbr (D146N) exhibits high thermostability and is frequently overexpressed to increase carbon flux from glucose toward phenylpyruvate and L-phenylalanine accumulation [350].
| Gene size: | |
| Protein size: | |
| Reactions | R576 |
| Compounds affected | 4-tyrosol , anthranilate , serotonin , L-tryptophan , psilocybin , L-tyrosine , deoxyviolacein , (indol-3-yl)acetate , 2-phenylethylamine , 5-hydroxy-L-tryptophan , 2-phenylethanol , catechol , shikimate , L-dopa , dopamine , genkwanin , L-phenylalanine , phenylethyl acetate , L-(-)-3-Phenyllactic acid and avenanthramide D |
Databases
| EraGene: | 2111676 |
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| UniProt: | P0AB91 |