Jung, Hwi‐Min; Han, Jae‐Ho & Oh, Min‐Kyu (2021). Improved production of 2,3‐butanediol and isobutanol by engineering electron transport chain in Escherichia coli. Microbial Biotechnology, 14(1), 213-226.

Strains

• Escherichia coli DSM01
• Escherichia coli ETC1
• Escherichia coli ETC2
• Escherichia coli ETC3
• Escherichia coli ETC4
• Escherichia coli DSM01‐BDO
• Escherichia coli ETC1‐BDO
• Escherichia coli ETC2‐BDO
• Escherichia coli ETC3‐BDO
• Escherichia coli ETC4‐BDO
• Escherichia coli ETC4‐IB
• Escherichia coli ETC3‐IB
• Escherichia coli ETC2‐IB
• Escherichia coli ETC1‐IB
• Escherichia coli UbiE117
• Escherichia coli UbiE86
• Escherichia coli UbiE26
• Escherichia coli UbiE117-BDO
• Escherichia coli UbiE86-BDO
• Escherichia coli UbiE26-BDO
• Escherichia coli UbiE117-IB
• Escherichia coli UbiE86-IB
• Escherichia coli UbiE26-IB
• Escherichia coli DSM01-IB

Plasmids

• pZADCB
• pBTBX‐2
• pBTDA
• pZS‐BDO

Metabolite production

• (R,R)-2,3-butanediol
  Titer
  • 2.45 g/L (Escherichia coli DSM01‐BDO in Flask)
  • 2.5 g/L (Escherichia coli UbiE117-BDO in Flask)
  • 2.14 g/L (Escherichia coli UbiE86-BDO in Flask)
  • 1.92 g/L (Escherichia coli UbiE26-BDO in Flask)
  • 2.75 g/L (Escherichia coli ETC1‐BDO in Flask)
  • 3.76 g/L (Escherichia coli ETC2‐BDO in Flask)
  • 3.0 g/L (Escherichia coli ETC3‐BDO in Flask)
  • 5.2 g/L (Escherichia coli ETC4‐BDO in Flask)
  • 3.59 g/L (Escherichia coli DSM01‐BDO in Batch)
  • 10.09 g/L (Escherichia coli DSM01‐BDO in Batch)
  • 17.71 g/L (Escherichia coli DSM01‐BDO in Batch)
  • 13.7 g/L (Escherichia coli ETC4‐BDO in Batch)
  • 14.73 g/L (Escherichia coli ETC4‐BDO in Batch)
  • 18.43 g/L (Escherichia coli ETC4‐BDO in Batch)
  • 14.8 g/L (Escherichia coli ETC4‐BDO in Batch)
  Substrate yield
  • 0.243 g/g of sustrate (Escherichia coli ETC4‐BDO in Batch)
  • 0.267 g/g of sustrate (Escherichia coli ETC4‐BDO in Batch)
  • 0.363 g/g of sustrate (Escherichia coli ETC4‐BDO in Batch)
  • 0.113 g/g of sustrate (Escherichia coli DSM01‐BDO in Batch)
  • 0.21 g/g of sustrate (Escherichia coli DSM01‐BDO in Batch)
  • 0.312 g/g of sustrate (Escherichia coli DSM01‐BDO in Batch)
• isobutanol
  Titer
  • 0.36 g/L (Escherichia coli DSM01-IB in Flask)
  • 1.12 g/L (Escherichia coli UbiE117-IB in Flask)
  • 1.94 g/L (Escherichia coli UbiE86-IB in Flask)
  • 2.22 g/L (Escherichia coli UbiE26-IB in Flask)
  • 1.87 g/L (Escherichia coli ETC1‐IB in Flask)
  • 2.54 g/L (Escherichia coli ETC2‐IB in Flask)
  • 2.1 g/L (Escherichia coli ETC3‐IB in Flask)
  • 2.87 g/L (Escherichia coli ETC4‐IB in Flask)