Lv, Huajun; Zhang, Ying; Shao, Jie; Liu, Haili & Wang, Yong. Ferulic acid production by metabolically engineered Escherichia coli. Bioresources and Bioprocessing. 2021, 8(1).
Strains
- Escherichia coli JM109 (DE3)
- Escherichia coli T5FA
- Escherichia coli p15aT5
- Escherichia coli T5FA + pntAB
- Escherichia coli p15aT5 + pntAB
- Escherichia coli p15aT5 + metK
- Escherichia coli p15aT5 + pntAB + metK
- Escherichia coli T5FA + pCL1920-T7
- Escherichia coli T5FA + icd
- Escherichia coli T5FA + gnd
- Escherichia coli T5FA + zwf
- Escherichia coli T5FA + gapN
Plasmids
- pBR322-T5-tal-sam5-comt
- p15a-T5-tal-sam5-comt
- pCL1920-T7
- pCL1920-T7-Ecicd
- pCL1920-T7-Ecgnd
- pCL1920-T7-Eczwf
- pCL1920-T7-EcpntAB
- pCL1920-T7-CagapN
- pCL1920-T7-SsmetK
- pCL1920-pntAB-metK
Metabolite production
-
ferulate
-
Titer
- 130.0 mg/L (Escherichia coli T5FA in Flask)
- 180.5 mg/L (Escherichia coli p15aT5 in Flask)
- 191.9 mg/L (Escherichia coli T5FA + pntAB in Flask)
- 207.3 mg/L (Escherichia coli p15aT5 + pntAB in Flask)
- 197.8 mg/L (Escherichia coli p15aT5 + metK in Flask)
- 212.5 mg/L (Escherichia coli p15aT5 + pntAB + metK in Flask)
- 140.0 mg/L (Escherichia coli T5FA + pCL1920-T7 in Flask)
- 121.7 mg/L (Escherichia coli T5FA + icd in Flask)
- 137.7 mg/L (Escherichia coli T5FA + gnd in Flask)
- 133.89 mg/L (Escherichia coli T5FA + zwf in Flask)
- 138.45 mg/L (Escherichia coli T5FA + gapN in Flask)
-
trans-caffeate
-
Titer
- 0.1 mg/L (Escherichia coli p15aT5 in Flask)
- 33.0 mg/L (Escherichia coli T5FA in Flask)
- 30.0 mg/L (Escherichia coli T5FA + pntAB in Flask)
- 13.0 mg/L (Escherichia coli p15aT5 + pntAB in Flask)
- 6.2 mg/L (Escherichia coli p15aT5 + metK in Flask)
- 11.9 mg/L (Escherichia coli p15aT5 + pntAB + metK in Flask)