Nybo, S. Eric; Saunders, Jacqueline & McCormick, Sean P. Metabolic engineering of Escherichia coli for production of valerenadiene. Journal of Biotechnology. 2017, 262, 60-66.

Strains

• Escherichia coli DH5αZ1
• Escherichia coli DH5αZ1 (pZE12MCS)
• Escherichia coli DH5αZ1 (pZE-wvds)
• Escherichia coli DH5αZ1 (pZE-wvds)/(pSTV-dxs-idi-fps)
• Escherichia coli DH5αZ1 (pZE-wvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
• Escherichia coli DH5αZ1 (pZE-cvds)
• Escherichia coli DH5αZ1 (pZE-cvds)/(pSTV-dxs-idi-fps)
• Escherichia coli DH5αZ1 (pZE-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
• Escherichia coli DH5αZ1 (pTrcHis-cvds)
• Escherichia coli DH5αZ1 (pTrcHis-cvds)/(pSTV-dxs-idi-fps)
• Escherichia coli DH5αZ1 (pTrcHis-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
• Escherichia coli BL21(DE3)/(pET-28a)
• Escherichia coli BL21(DE3)/(pET-wvds)
• Escherichia coli BL21(DE3)/(pET-wvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)
• Escherichia coli BL21(DE3)/(pET-cvds)
• Escherichia coli BL21(DE3)/(pET-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA)

Plasmids

• pZE12MCS
• pTrcHisA
• pSTV28
• pET-28a(+)
• pUC57-cvds
• pUC57-operon 3
• pZE-wvds
• pZE-cvds
• pTrcHis-cvds
• pSTV-dxs-idi-fps
• pET28a(+)-VoTPS1
• pET-cvds
• pBbA5c-MevT(CO)-MBIS(CO, ispA)

Metabolite production

• valerena-4,7(11)-diene
  Titer
  • 0.0 mg/L (Escherichia coli DH5αZ1 (pZE12MCS) in Flask)
  • 8.0 µg/L (Escherichia coli DH5αZ1 (pZE-wvds) in Flask)
  • 12.0 µg/L (Escherichia coli DH5αZ1 (pZE-wvds) in Flask)
  • 16.0 µg/L (Escherichia coli DH5αZ1 (pZE-cvds) in Flask)
  • 32.0 µg/L (Escherichia coli DH5αZ1 (pZE-cvds) in Flask)
  • 0.17 mg/L (Escherichia coli DH5αZ1 (pZE-wvds)/(pSTV-dxs-idi-fps) in Flask)
  • 2.09 mg/L (Escherichia coli DH5αZ1 (pZE-cvds)/(pSTV-dxs-idi-fps) in Flask)
  • 1.5 mg/L (Escherichia coli DH5αZ1 (pZE-wvds)/(pSTV-dxs-idi-fps) in Flask)
  • 11.0 mg/L (Escherichia coli DH5αZ1 (pZE-cvds)/(pSTV-dxs-idi-fps) in Flask)
  • 0.046 mg/L (Escherichia coli DH5αZ1 (pTrcHis-cvds) in Flask)
  • 20.3 mg/L (Escherichia coli DH5αZ1 (pTrcHis-cvds)/(pSTV-dxs-idi-fps) in Flask)
  • 42.5 mg/L (Escherichia coli DH5αZ1 (pTrcHis-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA) in Flask)
  • 0.345 mg/L (Escherichia coli BL21(DE3)/(pET-wvds) in Flask)
  • 1.09 mg/L (Escherichia coli BL21(DE3)/(pET-cvds) in Flask)
  • 10.5 mg/L (Escherichia coli BL21(DE3)/(pET-wvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA) in Flask)
  • 62.0 mg/L (Escherichia coli BL21(DE3)/(pET-cvds)/ (pBbA5c-MevT(CO)-MBIS(CO, ispA) in Flask)