Muñoz, Ana Joyce; Hernández-Chávez, Georgina; de Anda, Ramon; Martínez, Alfredo; Bolívar, Francisco & Gosset, Guillermo. Metabolic engineering of Escherichia coli for improving l-3,4-dihydroxyphenylalanine (l-DOPA) synthesis from glucose. Journal of Industrial Microbiology & Biotechnology. 2011, 38(11), 1845-1852.

Strains

• Escherichia coli W3110
• Escherichia coli VH33
• Escherichia coli W3110ΔtyrR
• Escherichia coli VH33ΔtyrR
• Escherichia coli W3110_TIR
• Escherichia coli VH33_TIR
• Escherichia coli W3110ΔtyrR_TIR
• Escherichia coli VH33ΔtyrR_TIR
• Escherichia coli W3110ΔtyrR_DOPA
• Escherichia coli VH33ΔtyrR_DOPA

Plasmids

• pTrc99A
• pJLBaroG^fbrtktA
• pTrctyrCpheA_CM
• pTrchpaBC
• pTrchpaBCTrctyrCpheA_CM

Metabolite production

• L-tyrosine
  Titer
  • 150.0 mg/L (Escherichia coli W3110_TIR in Flask)
  • 220.0 mg/L (Escherichia coli W3110ΔtyrR_TIR in Flask)
  • 220.0 mg/L (Escherichia coli VH33_TIR in Flask)
  • 150.0 mg/L (Escherichia coli VH33ΔtyrR_TIR in Flask)
  • 60.0 mg/L (Escherichia coli W3110ΔtyrR_DOPA in Flask)
  • 62.0 mg/L (Escherichia coli W3110ΔtyrR_DOPA in Flask)
  • 51.0 mg/L (Escherichia coli VH33ΔtyrR_DOPA in Flask)
  • 22.0 mg/L (Escherichia coli VH33ΔtyrR_DOPA in Flask)
  Substrate yield
  • 150.0 mg/g of sustrate (Escherichia coli W3110_TIR in Flask)
  • 42.0 mg/g of sustrate (Escherichia coli W3110ΔtyrR_TIR in Flask)
  • 118.0 mg/g of sustrate (Escherichia coli VH33_TIR in Flask)
  • 361.0 mg/g of sustrate (Escherichia coli VH33ΔtyrR_TIR in Flask)
• 3-dehydroshikimate
  Titer
  • 134.0 mg/L (Escherichia coli VH33_TIR in Flask)
  • 0.0 mg/L (Escherichia coli W3110ΔtyrR_DOPA in Flask)
  • 0.0 mg/L (Escherichia coli W3110ΔtyrR_DOPA in Flask)
  • 225.0 mg/L (Escherichia coli VH33ΔtyrR_DOPA in Flask)
  • 312.0 mg/L (Escherichia coli VH33ΔtyrR_DOPA in Flask)
• shikimate
  Titer
  • 340.0 mg/L (Escherichia coli VH33_TIR in Flask)
  • 248.0 mg/L (Escherichia coli W3110ΔtyrR_DOPA in Flask)
  • 228.0 mg/L (Escherichia coli W3110ΔtyrR_DOPA in Flask)
  • 226.0 mg/L (Escherichia coli VH33ΔtyrR_DOPA in Flask)
  • 271.0 mg/L (Escherichia coli VH33ΔtyrR_DOPA in Flask)
• L-dopa
  Titer
  • 235.0 mg/L (Escherichia coli W3110ΔtyrR_DOPA in Flask)
  • 269.0 mg/L (Escherichia coli W3110ΔtyrR_DOPA in Flask)
  • 313.0 mg/L (Escherichia coli VH33ΔtyrR_DOPA in Flask)
  • 320.0 mg/L (Escherichia coli VH33ΔtyrR_DOPA in Flask)
  • 1.51 g/L (Escherichia coli VH33ΔtyrR_DOPA in Batch)
  Substrate yield
  • 35.0 mg/g of sustrate (Escherichia coli W3110ΔtyrR_DOPA in Flask)
  • 41.0 mg/g of sustrate (Escherichia coli W3110ΔtyrR_DOPA in Flask)
  • 54.0 mg/g of sustrate (Escherichia coli VH33ΔtyrR_DOPA in Flask)
  • 54.0 mg/g of sustrate (Escherichia coli VH33ΔtyrR_DOPA in Flask)