Wei, Tao; Cheng, Bi-Yan & Liu, Jian-Zhong. Genome engineering Escherichia coli for L-DOPA overproduction from glucose. Scientific Reports. 2016, 6(1).
Strains
- Escherichia coli BW25113
- Escherichia coli AROM-1
- Escherichia coli AROM-2
- Escherichia coli AROM-3
- Escherichia coli AROM-4
- Escherichia coli TYR-1
- Escherichia coli DOPA-1
- Escherichia coli DOPA-2
- Escherichia coli BW25113 (pQE-hpaBC)
- Escherichia coli AROM-1 (pQE-hpaBC)
- Escherichia coli AROM-2 (pQE-hpaBC)
- Escherichia coli AROM-3 (pQE-hpaBC)
- Escherichia coli AROM-4 (pQE-hpaBC)
- Escherichia coli TYR-1 (pQE-hpaBC)
- Escherichia coli DOPA-30
- Escherichia coli DOPA-30N
- Escherichia coli DOPA-30 (pQE30)
- Escherichia coli DOPA-30 (pQE30-2hpaBC)
- Escherichia coli DOPA-30 (pQE30-phaBCN)
Plasmids
Metabolite production
-
L-dopa
-
Titer
- 138.7 mg/L (Escherichia coli BW25113 (pQE-hpaBC) in Flask)
- 148.3 mg/L (Escherichia coli AROM-1 (pQE-hpaBC) in Flask)
- 176.0 mg/L (Escherichia coli AROM-2 (pQE-hpaBC) in Flask)
- 173.9 mg/L (Escherichia coli AROM-3 (pQE-hpaBC) in Flask)
- 205.3 mg/L (Escherichia coli AROM-4 (pQE-hpaBC) in Flask)
- 209.2 mg/L (Escherichia coli TYR-1 (pQE-hpaBC) in Flask)
- 307.4 mg/L (Escherichia coli DOPA-1 in Flask)
- 210.2 mg/L (Escherichia coli DOPA-30 (pQE30) in Flask)
- 490.3 mg/L (Escherichia coli DOPA-30 (pQE30-2hpaBC) in Flask)
- 614.3 mg/L (Escherichia coli DOPA-30N in Flask)
- 650.3 mg/L (Escherichia coli DOPA-30 (pQE30-phaBCN) in Flask)
- 8.67 g/L (Escherichia coli DOPA-30N in Fed-Batch) Volumetric productivity
- 144.5 mg/L/h (Escherichia coli DOPA-30N in Fed-Batch) Substrate yield
- 62.7 mg/g of sustrate (Escherichia coli DOPA-30N in Fed-Batch)
-
L-tyrosine
-
Titer
- 292.5 mg/L (Escherichia coli BW25113 (pQE-hpaBC) in Flask)
- 263.5 mg/L (Escherichia coli AROM-1 (pQE-hpaBC) in Flask)
- 366.2 mg/L (Escherichia coli AROM-2 (pQE-hpaBC) in Flask)
- 304.0 mg/L (Escherichia coli AROM-3 (pQE-hpaBC) in Flask)
- 256.9 mg/L (Escherichia coli AROM-4 (pQE-hpaBC) in Flask)
- 256.6 mg/L (Escherichia coli TYR-1 (pQE-hpaBC) in Flask)
- 241.3 mg/L (Escherichia coli DOPA-1 in Flask)
- 462.7 mg/L (Escherichia coli DOPA-30 (pQE30) in Flask)
- 546.8 mg/L (Escherichia coli DOPA-30 (pQE30-2hpaBC) in Flask)
- 0.0 mg/L (Escherichia coli DOPA-30N in Flask)
- 0.0 mg/L (Escherichia coli DOPA-30 (pQE30-phaBCN) in Flask)