Li, Xiaoxi; Yu, Wenwen; Guo, Baoyuan; Lang, Xutao; Xu, Xianhao; Liu, Yanfeng; Li, Jianghua; Du, Guocheng; Lv, Xueqin & Liu, Long. Metabolic engineering of Escherichia coli for biosynthesis of inosinic acid. Systems Microbiology and Biomanufacturing. 2025, 5(4), 1609-1621.
Strains
- Escherichia coli BL21(DE3)
- Escherichia coli W3110
- Escherichia coli MG1655
- Escherichia coli GIMP10
- Escherichia coli GIMP11
- Escherichia coli GIMP12
- Escherichia coli AKN1
- Escherichia coli AKN2
- Escherichia coli AKN3
- Escherichia coli AKN4
- Escherichia coli AKN5
- Escherichia coli AKN6
- Escherichia coli AKN7
- Escherichia coli AKN8
- Escherichia coli AKN9
- Escherichia coli AKN10
- Escherichia coli AKN11
- Escherichia coli AKN12
- Escherichia coli AKN13
- Escherichia coli AKN14
- Escherichia coli AKN15
- Escherichia coli AKN16
- Escherichia coli AKN17
- Escherichia coli AKN18
- Escherichia coli AKN19
- Escherichia coli BL21(DE3) ΔpurR
- Escherichia coli BL21(DE3) prs
- Escherichia coli BL21(DE3) purF
- Escherichia coli BL21(DE3) purD
- Escherichia coli BL21(DE3) purN
- Escherichia coli BL21(DE3) purT
- Escherichia coli BL21(DE3) purL
- Escherichia coli BL21(DE3) purM
- Escherichia coli BL21(DE3) purK
- Escherichia coli BL21(DE3) purE
- Escherichia coli BL21(DE3) purC
- Escherichia coli BL21(DE3) purB
- Escherichia coli BL21(DE3) purH
- Escherichia coli P1
- Escherichia coli P2
- Escherichia coli P3
- Escherichia coli P4
- Escherichia coli P9
- Escherichia coli P10
- Escherichia coli P11
- Escherichia coli P12
- Escherichia coli P13
- Escherichia coli BL21(DE3) Δpgi
- Escherichia coli BL21(DE3) ΔpfkA
- Escherichia coli BL21(DE3) ΔpfkB
- Escherichia coli BL21(DE3) ΔpyrE
- Escherichia coli BL21(DE3) ΔhisG
- Escherichia coli BL21(DE3) ΔthiC
- Escherichia coli BL21(DE3) ΔyrfG
- Escherichia coli BL21(DE3) ΔnagD
- Escherichia coli BL21(DE3) ΔushA
- Escherichia coli BL21(DE3) ΔsurE
- Escherichia coli BL21(DE3) ΔpurR
- Escherichia coli BL21(DE3) ΔguaB
- Escherichia coli BL21(DE3) ΔpurA
Plasmids
- pACYDuet-prs
- pACYDuet-purF
- pACYDuet-purD
- pACYDuet-purN
- pACYDuet-purT
- pACYDuet-purL
- pACYDuet-purM
- pACYDuet-purK
- pACYDuet-purE
- pACYDuet-purC
- pACYDuet-purB
- pACYDuet-purH
- pJ07, PJ23119
- pETM6R1-EcCARBS22-KpcPARBS22-KpcPDRBS22
- pETM6R1-EcCARBS03-KpcPARBS07-KpcPDRBS22
- pETM6R1-EcCARBS03-KpcPA*RBS07-KpcPDRBS22
Metabolite production
-
IMP
-
Titer
- 434.2 mg/L (Escherichia coli BL21(DE3) in Flask)
- 484.6 mg/L (Escherichia coli AKN3 in Flask)
- 505.8 mg/L (Escherichia coli AKN4 in Flask)
- 562.0 mg/L (Escherichia coli AKN7 in Flask)
- 365.7 mg/L (Escherichia coli BL21(DE3) ΔpurR in Flask)
- 978.7 mg/L (Escherichia coli BL21(DE3) purF in Flask)
- 796.59 mg/L (Escherichia coli BL21(DE3) purC in Flask)
- 780.7 mg/L (Escherichia coli AKN10 in Flask)
- 654.5 mg/L (Escherichia coli AKN8 in Flask)
- 1409.6 mg/L (Escherichia coli AKN13 in Flask)
- 723.4 mg/L (Escherichia coli AKN14 in Flask)
- 1491.2 mg/L (Escherichia coli AKN15 in Flask)
- 1557.8 mg/L (Escherichia coli AKN16 in Flask)
- 2113.1 mg/L (Escherichia coli AKN17 in Flask)
- 3001.0 mg/L (Escherichia coli AKN17 in Fed-Batch)
- 1868.27 mg/L (Escherichia coli AKN18 in Flask)
- 1998.9 mg/L (Escherichia coli AKN19 in Flask) Biomass-specific yield
- 20.4 mg/OD600 (Escherichia coli AKN4 in Flask)
- 17.4 mg/OD600 (Escherichia coli BL21(DE3) in Flask)
- 22.5 mg/OD600 (Escherichia coli AKN7 in Flask)
- 23.8 mg/OD600 (Escherichia coli BL21(DE3) ΔpfkA in Flask)
- 29.8 mg/OD600 (Escherichia coli BL21(DE3) ΔguaB in Flask)
- 43.7 mg/OD600 (Escherichia coli BL21(DE3) ΔpurA in Flask)
- 59.7 mg/OD600 (Escherichia coli AKN16 in Flask)
- 81.6 mg/OD600 (Escherichia coli AKN17 in Flask) Substrate yield
- 0.015 g/g of sustrate (Escherichia coli AKN17 in Fed-Batch) Volumetric productivity
- 0.015 g/L/h (Escherichia coli AKN17 in Fed-Batch)
-
hypoxanthine
-
Titer
- 644.7 mg/L (Escherichia coli AKN15 in Flask)
- 527.2 mg/L (Escherichia coli AKN16 in Flask)
- 988.8 mg/L (Escherichia coli AKN17 in Fed-Batch)