Yang, Fengyu; Liao, Yaxin; Zhao, Zhenqiang; Cai, Mengmeng; You, Jiajia; Qiao, Zhina; Xu, Meijuan & Rao, Zhiming. Development of Escherichia coli by combining channel engineering and energy engineering for the production of L-histidine. Chemical Engineering Journal. 2025, 520, 165521.

Strains

• Escherichia coli HIS1-1
• Escherichia coli HIS1-2
• Escherichia coli HIS1-3
• Escherichia coli HIS1-4
• Escherichia coli HIS1-5
• Escherichia coli HIS1-6
• Escherichia coli HIS1-7
• Escherichia coli HIS1-8
• Escherichia coli HIS1-9
• Escherichia coli HIS2-1
• Escherichia coli HIS2-2
• Escherichia coli HIS2-3
• Escherichia coli HIS2-4
• Escherichia coli HIS2-5
• Escherichia coli HIS2-6
• Escherichia coli HIS3-1
• Escherichia coli HIS3-2
• Escherichia coli HIS3-3
• Escherichia coli HIS3-4
• Escherichia coli HIS3-5
• Escherichia coli HIS3-6
• Escherichia coli HIS3-7
• Escherichia coli HIS3-8
• Escherichia coli HIS3-9
• Escherichia coli HIS3-10
• Escherichia coli HIS3-11
• Escherichia coli HIS3-12
• Escherichia coli HIS3-13
• Escherichia coli HIS4-1
• Escherichia coli HIS4-2
• Escherichia coli HIS4-3
• Escherichia coli HIS5-1
• Escherichia coli HIS5-2
• Escherichia coli HIS5-3
• Escherichia coli HIS5-4
• Escherichia coli HIS5-5
• Escherichia coli HIS5-6
• Escherichia coli HIS5-7
• Escherichia coli HIS5-8
• Escherichia coli HIS5-9
• Escherichia coli HIS5-10
• Escherichia coli HIS5-11
• Escherichia coli HIS5-12

Metabolite production

• L-histidine
  Titer
  • 0.12 g/L (Escherichia coli HIS1-1 in Flask)
  • 0.32 g/L (Escherichia coli HIS1-2 in Flask)
  • 1.0 g/L (Escherichia coli HIS1-4 in Flask)
  • 0.86 g/L (Escherichia coli HIS1-3 in Flask)
  • 0.36 g/L (Escherichia coli HIS1-5 in Flask)
  • 0.4 g/L (Escherichia coli HIS1-6 in Flask)
  • 2.55 g/L (Escherichia coli HIS1-8 in Flask)
  • 2.79 g/L (Escherichia coli HIS2-3 in Flask)
  • 13.02 g/L (Escherichia coli HIS2-3 in Fed-Batch)
  • 2.84 g/L (Escherichia coli HIS3-1 in Flask)
  • 3.16 g/L (Escherichia coli HIS3-2 in Flask)
  • 3.83 g/L (Escherichia coli HIS3-3 in Flask)
  • 4.53 g/L (Escherichia coli HIS3-4 in Flask)
  • 4.95 g/L (Escherichia coli HIS3-5 in Flask)
  • 5.52 g/L (Escherichia coli HIS3-6 in Flask)
  • 5.74 g/L (Escherichia coli HIS3-7 in Flask)
  • 5.97 g/L (Escherichia coli HIS3-8 in Flask)
  • 6.11 g/L (Escherichia coli HIS3-9 in Flask)
  • 7.28 g/L (Escherichia coli HIS3-12 in Flask)
  • 40.6 g/L (Escherichia coli HIS3-12 in Fed-Batch)
  • 7.82 g/L (Escherichia coli HIS4-1 in Flask)
  • 8.21 g/L (Escherichia coli HIS4-2 in Flask)
  • 8.48 g/L (Escherichia coli HIS5-3 in Flask)
  • 8.69 g/L (Escherichia coli HIS5-7 in Flask)
  • 8.91 g/L (Escherichia coli HIS5-8 in Flask)
  • 9.42 g/L (Escherichia coli HIS5-11 in Flask)
  • 52.32 g/L (Escherichia coli HIS5-11 in Fed-Batch)
  Substrate yield
  • 0.06 g/g of sustrate (Escherichia coli HIS2-3 in Fed-Batch)
  • 0.18 g/g of sustrate (Escherichia coli HIS3-12 in Fed-Batch)
  • 0.22 g/g of sustrate (Escherichia coli HIS5-11 in Fed-Batch)
  Volumetric productivity
  • 0.43 g/L/h (Escherichia coli HIS2-3 in Fed-Batch)
  • 1.13 g/L/h (Escherichia coli HIS3-12 in Fed-Batch)
  • 1.45 g/L/h (Escherichia coli HIS5-11 in Fed-Batch)
• L-glutamate
  Titer
  • 2.49 g/L (Escherichia coli HIS4-3 in Flask)
• acetate
  Titer
  • 1.9 g/L (Escherichia coli HIS5-11 in Fed-Batch)