Guo, Cui; Luu, Nguyen N.T.; Adwer, Maryem M.; Hosseinzadeh, Hemen; Balan, Venkatesh; Yan, Yajun & Lin, Yuheng. Engineering Non-Natural Biosynthetic Pathways for Efficient Microbial Production of Psilocybin and Psilocin. Metabolic Engineering. 2025.
Strains
- Escherichia coli BW25113ΔtnaA
- Escherichia coli BW25113ΔtnaA (F´)
- Escherichia coli PP01
- Escherichia coli PP02
- Escherichia coli PP03
- Escherichia coli PP04
- Escherichia coli PP05
- Escherichia coli PP06
- Escherichia coli PP07
- Escherichia coli PP08
- Escherichia coli PP09
- Escherichia coli PP10
- Escherichia coli PP11
- Escherichia coli PP12
- Escherichia coli PP13
- Escherichia coli PP14
- Escherichia coli PP15
- Escherichia coli PP16
- Escherichia coli PP17
- Escherichia coli PP18
- Escherichia coli PP19
- Escherichia coli PP20
- Escherichia coli PP22
- Escherichia coli PP23
- Escherichia coli PP25
- Escherichia coli PP26
- Escherichia coli PP27
- Escherichia coli PP28
Plasmids
- pHA-GFP
- pHA-OhmK
- pHA-Arg5
- pLC-GFP
- pLC-ChuA
- pHA-Arg5-NdDDC
- pHA-Arg5-DoDDC
- pHA-Arg5-KvDDC
- pMK-GFP
- pMK-PcPsiM-PcPsiK
- pMK-PanPsiM-PcPsiK
- pHA-AD-MK
- pHA-AD-M
- pMK-PcPsiK
- pHA-ADK-M
- pMK-RmNMT
- pMK-CcNMT
- pMK-CbNMT
- pMK-LbNMT
- pHA-AD-N
- pHA-ADN
- pHA-NAD
- pHA-RmNMT
- pMK-AD
- pMK-MetK
- pLC-YhjV
- pMK-T-V
- pLC-TrpEfbrDCBA
- pMK-LPTG
- pMK-LPTG-K
- pLC-TrpEfbrDCBA-LPTG
Metabolite production
-
4-hydroxy-L-tryptophan
-
Titer
- 149.29 mg/L (Escherichia coli PP02 in Flask)
- 0.0 mg/L (Escherichia coli PP01 in Flask)
- 220.72 mg/L (Escherichia coli PP02 in Flask)
- 175.59 mg/L (Escherichia coli PP03 in Flask)
- 761.66 mg/L (Escherichia coli PP02 in Flask)
- 920.7 mg/L (Escherichia coli PP06 in Flask)
- 193.33 mg/L (Escherichia coli PP04 in Flask)
- 539.1 mg/L (Escherichia coli PP05 in Flask)
- 0.0 mg/L (Escherichia coli PP27 in Flask)
- 0.0 mg/L (Escherichia coli PP27 in Fed-Batch)
-
tryptamine
-
Titer
- 5.71 mg/L (Escherichia coli PP04 in Flask)
- 9.84 mg/L (Escherichia coli PP05 in Flask)
- 64.65 mg/L (Escherichia coli PP06 in Flask)
-
psilocybin
-
Titer
- 562.9 mg/L (Escherichia coli PP07 in Flask)
- 393.22 mg/L (Escherichia coli PP08 in Flask)
- 700.0 mg/L (Escherichia coli PP09 in Flask)
- 776.36 mg/L (Escherichia coli PP10 in Flask)
- 639.0 mg/L (Escherichia coli PP11 in Flask)
- 475.88 mg/L (Escherichia coli PP27 in Flask)
- 423.56 mg/L (Escherichia coli PP26 in Flask)
- 220.65 mg/L (Escherichia coli PP27 in Flask)
- 557.91 mg/L (Escherichia coli PP27 in Flask)
- 2.0 g/L (Escherichia coli PP27 in Fed-Batch) Volumetric productivity
- 26.7 mg/L/h (Escherichia coli PP27 in Fed-Batch)
-
psilocin
-
Titer
- 33.01 mg/L (Escherichia coli PP12 in Flask)
- 0.0 mg/L (Escherichia coli PP15 in Flask)
- 0.0 mg/L (Escherichia coli PP14 in Flask)
- 0.0 mg/L (Escherichia coli PP13 in Flask)
- 115.2 mg/L (Escherichia coli PP18 in Flask)
- 119.1 mg/L (Escherichia coli PP20 in Flask)
- 163.46 mg/L (Escherichia coli PP22 in Flask)
- 187.96 mg/L (Escherichia coli PP23 in Flask)
- 105.49 mg/L (Escherichia coli PP28 in Flask)
- 43.83 mg/L (Escherichia coli PP28 in Flask)
- 148.97 mg/L (Escherichia coli PP28 in Flask)
-
L-tryptophan
-
Titer
- 735.23 mg/L (Escherichia coli PP25 in Flask)
- 577.9 mg/L (Escherichia coli PP28 in Flask)
- 191.18 mg/L (Escherichia coli PP27 in Flask)
- 1.07 g/L (Escherichia coli PP27 in Fed-Batch)
-
anthranilate
-
Titer
- 490.11 mg/L (Escherichia coli PP27 in Flask)
- 2.0 g/L (Escherichia coli PP27 in Flask)
-
4-hydroxytryptamine
-
Titer
- 0.0 mg/L (Escherichia coli PP27 in Fed-Batch)