Kawano, Yusuke; Onishi, Fumito; Shiroyama, Maeka; Miura, Masashi; Tanaka, Naoyuki; Oshiro, Satoshi; Nonaka, Gen; Nakanishi, Tsuyoshi & Ohtsu, Iwao. Improved fermentative l-cysteine overproduction by enhancing a newly identified thiosulfate assimilation pathway in Escherichia coli. Applied Microbiology and Biotechnology. 2017, 101(18), 6879-6889.

Strains

• Escherichia coli BW25113
• Escherichia coli JW2414
• Escherichia coli JW2407
• Escherichia coli ΔcysMΔglpE
• Escherichia coli ΔcysM/pCA24N
• Escherichia coli ΔcysM/pTusD
• Escherichia coli ΔcysM/pTusA
• Escherichia coli ΔcysM/pYibN
• Escherichia coli ΔcysM/pSseA
• Escherichia coli ΔcysM/pYceA
• Escherichia coli ΔcysM/pGlpE
• Escherichia coli WT/pDES/pCA24N
• Escherichia coli WT/pDES/pGlpE
• Escherichia coli ΔcysM/pDES/pCA24N
• Escherichia coli ΔcysM/pDES/pGlpE

Plasmids

• pCA24N
• pGlpE
• pQB2
• pYceA
• pSseA
• pYibN
• pTusA
• pTusD
• pDES

Metabolite production

• L-cysteine
  Titer
  • 1529.0 mg/L (Escherichia coli WT/pDES/pGlpE in Flask)
  • 1018.67 mg/L (Escherichia coli WT/pDES/pCA24N in Flask)
  • 686.73 mg/L (Escherichia coli WT/pDES/pCA24N in Flask)
  • 1205.39 mg/L (Escherichia coli WT/pDES/pGlpE in Flask)
  • 55.81 mg/L (Escherichia coli ΔcysM/pDES/pCA24N in Flask)
  • 63.07 mg/L (Escherichia coli ΔcysM/pDES/pCA24N in Flask)
  • 135.68 mg/L (Escherichia coli ΔcysM/pDES/pGlpE in Flask)
  • 111.83 mg/L (Escherichia coli ΔcysM/pDES/pGlpE in Flask)