Escherichia coli T6-47-6
This strain does not exhibit an improved production of the intended metabolite when compared with its closest ancestors.
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | AHVhr AECr MFr Rifr Metl Thr− | Plasmid pEC-XK99E-tdhEc-aaoSo- PnoxELc added | Plasmid pCDFDuet-Psod-sstTEc addedBars (|) indicate differences between strains.
Plasmids
-
pEC-XK99E-tdhEc-aaoSo- PnoxELc (Ptrc-tdhEc-aaoSo, Ptrc-noxELc)
pCDFDuet-Psod-sstTEc (Psod-sstTEc)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
|
Overexpression
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| 2,5-Dimethylpyrazine | Titer | 0.5 g/L * | Flask | [358] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.