Escherichia coli T6-47

The T6-47 strain lacks a functional tdh gene, thereby avoiding the conversion of threonine into aminoacetone and consequently preventing the formation of 2,5-dimethylpyrazine.

This strain does not exhibit an improved production of the intended metabolite when compared with its closest ancestors.

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli MG1655

Escherichia coli T6-47

Derived strains

Genotype with respect to parental

AHV^hr AEC^r MF^r Rif^r Met^l Thr⁻

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | AHV^hr AEC^r MF^r Rif^r Met^l Thr⁻

Bars (|) indicate differences between strains.

Production

Metabolites	Production type	Production	Carbon source	Time	Scale	Ref.
L-threonine	Titer	11.2 g/L	Glucose	48 h	Flask	[358]
2,5-Dimethylpyrazine	Titer	0.0 mg/L	Glucose	48 h	Flask	[358]
2,5-Dimethylpyrazine	Titer	0.0 mg/L	Glucose L-threonine	48 h	Flask	[358]
aminoacetone	Titer	0.0 g/L	Glucose	48 h	Flask	[358]
aminoacetone	Titer	0.0 g/L	Glucose tyramine	48 h	Flask	[358]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Xu, Jianzhong; Yu, Haibo; Chen, Xiulai; Liu, Liming & Zhang, Weiguo (2020). Accelerated Green Process of 2,5-Dimethylpyrazine Production from Glucose by Genetically Modified Escherichia coli. ACS Synthetic Biology, 9(9), 2576-2587.