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pN-3

Ancestors
  • All plasmids
    • pET-28a(+)
      • pN-3



Main features
f1 ori, pBR322 ori, lacI, PT7, aldMs

Selection marker:

Kanamycin



Modifications
Gene Organism Modification
ald
Mycobacterium smegmatis
Overexpression



Strains harboring plasmid pN-3
  • Escherichia coli BL21(DE3)/pN-3

References
  • Pengfei Gu, Qianqian Ma, Shuo Zhao, Qiang Li & Juan Gao (2023). Alanine dehydrogenases from four different microorganisms: characterization and their application in L-alanine production. Biotechnology for Biofuels and Bioproducts.

Reference

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