pET-28a(+)

Ancestors

All plasmids

pET-28a(+)

Derivated

Main features

f1 ori, pBR322 ori, lacI, P_T7, N-terminal His•Tag®/thrombin/ T7•Tag®

Selection marker:

Kanamycin

Strains harboring plasmid pET-28a(+)

Escherichia coli BL21(DE3)/(pET-28a)

References

Long M, Xu M, Ma Z, Pan X, You J, Hu M, Shao Y, Yang T, Zhang X, Rao Z. Significantly enhancing production of trans-4-hydroxy-l-proline by integrated system engineering in Escherichia coli. Sci Adv. 2020 May 22;6(21):eaba2383.
Hao Zhang, Zhong Liang, Ming Zhao, Yanqin Ma, Zhengshan Luo, Sha Li & Hong Xu (2022). Metabolic Engineering of Escherichia coli for Ectoine Production With a Fermentation Strategy of Supplementing the Amino Donor. Frontiers in Bioengineering and Biotechnology.
Li, Hua; Wang, Baoshi; Zhu, Linghuan; Cheng, Shi; Li, Youran; Zhang, Liang; Ding, Zhong Yang; Gu, Zheng Hua & Shi, Gui Yang. Metabolic engineering of Escherichia coli W3110 for L-homoserine production. Process Biochemistry. 2016, 51(12), 1973-1983.
Zhou, Wei; Bi, Huiping; Zhuang, Yibin; He, Qinglin; Yin, Hua; Liu, Tao & Ma, Yanhe. Production of Cinnamyl Alcohol Glucoside from Glucose in Escherichia coli. Journal of Agricultural and Food Chemistry. 2017, 65(10), 2129-2135.
Jiaping Wang, Cong Gao, Xiulai Chen & Liming Liu (2021). Engineering the Cad pathway in Escherichia coli to produce glutarate from l-lysine. Applied Microbiology and Biotechnology.
Nybo, S. Eric; Saunders, Jacqueline & McCormick, Sean P. Metabolic engineering of Escherichia coli for production of valerenadiene. Journal of Biotechnology. 2017, 262, 60-66.