Escherichia coli BL21(DE3)

The pgl gene is absent in Escherichia coli BL21(DE3), leading to reduced efficiency of the pentose phosphate pathway (PPP), diminished NADPH generation, lower availability of Ru5P and erythrose-4-phosphate, and accumulation of γ/δ-6-phosphogluconolactone [345].

Synonyms

Escherichia coli BTY0

Escherichia coli B1

Ancestors

Derived strains

Genotype with respect to parental

F− ompT hsdSB (rB−mB−) gal dcm λ(DE3)


Genotype with respect to wild type
F− dcm gal ompT hsdS(rB- mB-) | F− ompT hsdSB (rB−mB−) gal dcm λ(DE3)

Bars (|) indicate differences between strains.


Production
Metabolites Production type Production Biomass Carbon source Time Scale Ref.
malonyl-CoA Biomass-specific yield 0.07 nmol/mg DCW Flask [198]
acetyl-CoA Biomass-specific yield 0.93 nmol/mg DCW Flask [198]
IMP Titer 434.2 mg/L Flask [341]
IMP Biomass-specific yield 17.4 mg/OD600 Flask [341]

* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References