Escherichia coli HA-6
This strain does not exhibit an improved production of the intended metabolite when compared with its closest ancestors.
Ancestors
Genotype with respect to wild type
F− dcm gal ompT hsdS(rB- mB-) | F− ompT hsdSB (rB−mB−) gal dcm λ(DE3) | Plasmid pC-Os4CL-HCBT added | Plasmid pA-aroGfbr-ppsA-tktA-SeTAL-tyrAfbr added | Plasmid pE-trpEG addedBars (|) indicate differences between strains.
Plasmids
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pA-aroGfbr-ppsA-tktA-SeTAL-tyrAfbr (TALSe, aroGfbr, ppsA, tktA, tyrAfbr)
pC-Os4CL-HCBT (Os4CL, HCBT)
pE-trpEG (trpEG)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Overexpression
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Feedback insensitive
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Overexpression
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Overexpression
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Feedback insensitive
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| avenanthramide D | Titer | 25.0 mg/L | Flask | [360] | |||
| 4-coumarate | Titer | 105.45 mg/L * | Flask | [360] | |||
| anthranilate | Titer | 0.9 mg/L * | Flask | [360] | |||
| L-tyrosine | Titer | 527.49 mg/L * | Flask | [360] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.