Escherichia coli HA-4

Ancestors

Genotype with respect to wild type

F⁻ dcm gal ompT hsdS(r_B^- m_B^-) | F⁻ ompT hsdS_B (r_B⁻m_B⁻) gal dcm λ(DE3) | Plasmid pC-Os4CL-HCBT added | Plasmid pE-trpEG added | Plasmid pA-aroG-SeTAL-tyrA added

Bars (|) indicate differences between strains.

Plasmids

pA-aroG-SeTAL-tyrA (TAL_Se, aroG_Ec, tyrA_Ec)

pC-Os4CL-HCBT (Os4CL, HCBT)

pE-trpEG (trpEG)

Modifications

Origin	Gene	Organism	Modification
pA-aroG-SeTAL-tyrA	sam8	Saccharothrix espanaensis	Overexpression
pA-aroG-SeTAL-tyrA	aroG	Escherichia coli	Overexpression
pA-aroG-SeTAL-tyrA	tyrA	Escherichia coli	Overexpression
pC-Os4CL-HCBT	4CL	Oryza sativa	Overexpression
pC-Os4CL-HCBT	HCBT2	Dianthus caryophyllus	Overexpression
pE-trpEG	trpE	Escherichia coli	Overexpression

Production

Metabolites	Production type	Production	Carbon source	Scale	Ref.
avenanthramide D	Titer	74.9 mg/L	Glucose	Flask	[360]
4-coumarate	Titer	2.52 mg/L ^*	Glucose	Flask	[360]
anthranilate	Titer	12.17 mg/L ^*	Glucose	Flask	[360]
L-tyrosine	Titer	4.08 mg/L ^*	Glucose	Flask	[360]

* Inferred from plots using RetroPlot.

Heterologous.

Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.

References

Lee, Su Jin; Sim, Geun Young; Kang, Hyunook; Yeo, Won Seok; Kim, Bong-Gyu & Ahn, Joong-Hoon (2018). Synthesis of avenanthramides using engineered Escherichia coli. Microbial Cell Factories, 17(1).