H⁺ + menaquinol-8 + fumarate → menaquinone-8 + succinate (R6531)
- Silencing
  - Genome
NAD⁺ + (R)-lactate ← NADH + pyruvate + H⁺ (R504)
- Silencing
  - Genome
H₂O + pyruvate + ubiquinone-8 → acetate + ubiquinol-8 + CO₂ (R4535)
- Silencing
  - Genome
acetyl-CoA + phosphate ↔ coenzyme A + acetyl phosphate (R424)
- Silencing
  - Genome
acetate + ATP ↔ acetyl phosphate + ADP (R45)
- Silencing
  - Genome
ethanol + NAD⁺ ↔ acetaldehyde + NADH + H⁺ (R157)
- Silencing
  - Genome
coenzyme A + acetaldehyde + NAD⁺ ↔ NADH + H⁺ + acetyl-CoA (R156)
- Silencing
  - Genome
pyruvate + hydrogencarbonate + ATP → H⁺ + phosphate + oxaloacetate + ADP (R754)
- Feedback insensitive
  - Genome
H₂O + acetyl-CoA + oxaloacetate → coenzyme A + H⁺ + citrate (R92)
- Overexpression
  - Genome
D-threo-isocitrate ↔ glyoxylate + succinate (R1019)
- Silencing
  - Genome
    - Escherichia coli W3110-TAA08
trans-aconitate ↔ cis-aconitate (R3928)
- Overexpression
  - Plasmid
H⁺ + trans-aconitate → itaconate + CO₂ (R9070)
- Overexpression
  - Plasmid
NADP⁺ + D-threo-isocitrate → NADPH + 2-oxoglutarate + CO₂ (R384)
- Silencing
  - Genome
    - Escherichia coli W3110-TAA07