L-aspartate + ATP → L-aspartyl-4-phosphate + ADP (R295)
- Feedback insensitive
  - Genome
  - Plasmid
- Overexpression
  - Genome
  - Plasmid
L-homoserine + NAD(P)+ ← L-aspartate 4-semialdehyde + NAD(P)H + H⁺ (R234)
- Feedback insensitive
  - Genome
  - Plasmid
- Overexpression
  - Genome
  - Plasmid
L-homoserine + ATP → O-phospho-L-homoserine + H⁺ + ADP (R153)
- Overexpression
  - Genome
  - Plasmid
H₂O + O-phospho-L-homoserine → L-threonine + phosphate (R646)
- Overexpression
  - Genome
  - Plasmid
NAD⁺ + L-threonine → H⁺ + L-2-amino-3-oxobutanoate + NADH (R915)
- Silencing
  - Genome
L-threonine + H⁺ → L-threonine + H⁺ (R00835)
- Silencing
  - Genome
L-threonine + H⁺ → L-threonine + H⁺ (R00817)
- Overexpression
  - Genome
  - Plasmid
L-threonine + Na⁺ ↔ L-threonine + Na⁺ (R00821)
- Silencing
  - Genome
    - Escherichia coli MG-105
    - Escherichia coli MDS-205
H⁺ + meso-diaminopimelate → L-lysine + CO₂ (R367)
- Silencing
  - Genome
L-homoserine + succinyl-CoA → coenzyme A + O-succinyl-L-homoserine (R369)
- Silencing
  - Genome
L-threonine → 2-oxobutanoate + ammonium (R1249)
- Attenuation
  - Genome
phosphate + oxaloacetate ↔ phosphoenolpyruvate + hydrogencarbonate (R390)
- Overexpression
  - Genome
coenzyme A + acetate + ATP ↔ acetyl-CoA + diphosphate + AMP (R1142)
- Overexpression
  - Genome
    - Escherichia coli TH28C (pBRThrABCR3)
phosphoenolpyruvate + β-D-glucopyranose → β-D-glucose 6-phosphate + pyruvate (R4216)
- Silencing
  - Genome
    - Escherichia coli P_2.1-2901ΔptsG